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Immunology and Infection

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辅助性T细胞作为一种遗传方法来研究逆转录病毒转导机制控制其分化和功能

JoVE Journal
Immunology and Infection

This content is Free Access.

JoVE Journal Immunology and Infection

Retroviral Transduction of Helper T Cells as a Genetic Approach to Study Mechanisms Controlling their Differentiation and Function

Please note that all translations are automatically generated. Click here for the English version.

辅助性T细胞作为一种遗传方法来研究逆转录病毒转导机制控制其分化和功能

DOI:

10.3791/54698-v

•

11:50 min

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November 04, 2016

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Yogesh Singh², Oliver A. Garden, Florian Lang, Bradley S. Cobb

¹Department of Comparative Biomedical Sciences,The Royal Veterinary College, ²Institute of Physiology I, Cardiology & Vascular Medicine,Eberhard Karls University of Tuebingen, ³Department of Clinical Science and Services,The Royal Veterinary College

Chapters

00:05Title
01:01Transfection by Calcium Phosphate Precipitation and Virus Collection
03:02Isolation of Leukocyte Cells
04:13Isolation of CD4⁺ T Cells Using Magnetic Beads Kit
06:02Isolation of Naïve CD4⁺ T Cells Using Cell Separation Columns
08:10Retroviral Transduction
09:37Results: Analysis of Retrovirus-transfected HEK 293 Cells and Retrovirus Transduced Helper T Cells
11:16Conclusion

Summary

Automatic Translation

许多实验系统已被利用来理解调节T细胞的发育和功能中的免疫应答的机制。这里使用逆转录病毒转导的基因的方法的描述，这是经济，时间效率，最重要的是，高度信息识别调控通路。

Tags

Keywords: Retroviral Transduction Helper T Cells Genetic Approach T Cell Differentiation T Cell Function MicroRNA Regulation HEK293T Cells Calcium Phosphate Transfection

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