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Journal / Immunology and Infection / A Simple Fluorescens Assay for Kvantificering af…
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Immunology and Infection

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JoVE Journal Immunology and Infection
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A Simple Fluorescens Assay for Kvantificering af Canine Neutrofil Ekstracellulær Trap Frigivelse

Article DOI: 10.3791/54726 • 06:45 min
November 21st, 2016 •
1, 2, 3
1Department of Veterinary Microbiology and Preventative Medicine, College of Veterinary Medicine, Iowa State University, 2MRC Centre for Inflammation Research, University of Edinburgh, 3Department of Veterinary Clinical Sciences, College of Veterinary Medicine, Iowa State University

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November 21st, 2016

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Neutrofile ekstracellulære fælder (net) er netværk af DNA, histoner og neutrofile proteiner. Selv om en del af den medfødte immunreaktion, er NET impliceret i autoimmunitet og trombose. Denne protokol beskriver en enkel fremgangsmåde til hunde neutrofil isolering og kvantificering af NET bruger mikroplade fluorescens-assay.

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