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Functional Genomics Screening Verwendung zur Identifizierung möglicher neuer Wirkstoff-Targets in Cancer Cell Sphäroid-Kulturen
JoVE Journal
Cancer Research
This content is Free Access.
JoVE Journal Cancer Research
Utilizing Functional Genomics Screening to Identify Potentially Novel Drug Targets in Cancer Cell Spheroid Cultures
DOI:

07:48 min

December 26, 2016

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Chapters

  • 00:05Title
  • 01:17Reverse Transfection of BT474 Cells
  • 03:51Automated Image Acquisition
  • 05:23Results: Functional Genomic Investigation of BT474 Spheroids Uncovers Oncogenic Dependencies
  • 07:02Conclusion

Summary

Automatic Translation

Identifying novel drug targets that transition from pre-clinical testing to human trials is a scientific priority. To that end, here we describe a functional genomics approach for examining the impact of gene depletion on cancer cell line spheroids, which more appropriately model human cancers in vivo.

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