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Journal
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Genetics
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光遗传学随机突变用组蛋白miniSOG在<em>℃。线虫</em
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JoVE Journal
Genetics
Optogenetic Random Mutagenesis Using Histone-miniSOG in
C. elegans
Please note that all translations are automatically generated.
Click here for the English version.
光遗传学随机突变用组蛋白miniSOG在<em>℃。线虫</em
DOI:
10.3791/54810-v
•
04:51 min
•
November 14, 2016
•
Kentaro Noma
,
Yishi Jin
2
1
Division of Biological Sciences, Section of Neurobiology
,
University of California in San Diego and Howard Hughes Medical Institute
,
2
Department of Cellular and Molecular Medicine
,
University of California in San Diego School of Medicine and Howard Hughes Medical Institute
Chapters
00:05
Title
00:46
Assembling the LED Illuminator
01:52
Blue Light Treatment
03:46
Results: Screen Mutation Rate
04:27
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
基因编码的蛋白，miniSOG诱导蓝光依赖性全基因组的遗传突变。这种突变方法简单，快速，免费的有毒化学物质，并非常适用于正向遗传学筛查和转基因整合。
Tags
Keywords: Optogenetic
Random Mutagenesis
Histone-miniSOG
C. Elegans
LED
Reactive Oxygen Species
ROS-generating Proteins
Mutagenesis Protocol
Blue Light
Digital Function Generator
Photometer
Copper Chloride
Sine Wave
Young Adult Hermaphrodites
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