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Neuroscience
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脳血管内皮細胞での生体エネルギー機能の評価
JoVE Journal
Neuroscience
This content is Free Access.
JoVE Journal
Neuroscience
Evaluation of Bioenergetic Function in Cerebral Vascular Endothelial Cells
Please note that all translations are automatically generated.
Click here for the English version.
脳血管内皮細胞での生体エネルギー機能の評価
DOI:
10.3791/54847-v
•
06:15 min
•
November 19, 2016
•
Stephanie L. Rellick
2,4
,
Heng Hu
3,4
,
James W. Simpkins
4
,
Xuefang Ren
3,4
1
Department of Physiology and Pharmacology
,
West Virginia University
,
2
Mitochondrial Evaluation Core
,
West Virginia University
,
3
Experimental Stroke Core
,
West Virginia University
,
4
Center for Basic and Translational Stroke Research
,
West Virginia University
Chapters
00:05
Title
00:36
Cerebral Vascular Endothelial (CVE) Cell Culture and Passage
01:48
Mitochondrial Functional Assessment
03:27
Results: Representative CVE Bioenergetic Function Analyses
05:10
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
内皮細胞のミトコンドリアは、血液脳関門の完全性を維持するために重要です。私たちは、脳血管内皮細胞に生体エネルギー機能を測定するためのプロトコルを紹介します。
Tags
Mitochondrial Function
Neurological Disorders
Blood-brain Barrier
Cerebral Vascular Endothelial Cells
Extracellular Flux
Cell Culture
Cell Medium
Trypsin EDTA
Cell Seeding
Cell Attachment
Treatment
Mitochondrial Functional Assessment
Extracellular Flux Sensor Cartridge
Extracellular Flux Assay Medium
Bioanalyzer
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