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Journal
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Biochemistry
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一种优化的协议电泳迁移分析使用红外荧光染料标记的寡核苷酸
JoVE Journal
Biochemistry
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JoVE Journal
Biochemistry
An Optimized Protocol for Electrophoretic Mobility Shift Assay Using Infrared Fluorescent Dye-labeled Oligonucleotides
Please note that all translations are automatically generated.
Click here for the English version.
一种优化的协议电泳迁移分析使用红外荧光染料标记的寡核苷酸
DOI:
10.3791/54863-v
•
09:58 min
•
November 29, 2016
•
Yi-Wen Hsieh
,
Amel Alqadah
,
Chiou-Fen Chuang
1
Department of Biological Sciences
,
University of Illinois at Chicago
Chapters
00:05
Title
00:32
Preparation of Gel, Infrared Fluorescent Dye-labeled Probes and Unlabeled Probes
04:49
Binding Reaction and Electrophoresis
06:37
Imaging
07:35
Results: Electrophoretic Mobility Shift Assays Using Purified Proteins and Dye-labeled DNA Probes
08:57
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
我们在这里描述使用纯化SOX-2蛋白与红外荧光染料标记的DNA探针作为案例研究解决的重要生物学问题一起荧光电泳迁移实验(FEMSA)的优化方案。
Tags
Electrophoretic Mobility Shift Assay
Infrared Fluorescent Dye
Oligonucleotides
Protein-DNA Interactions
Molecular Biology
Biochemistry
Non-radioactive Probes
Native Polyacrylamide Gel
TBE Buffer
Glycerol
Annealing
Double-stranded DNA Probes
Klenow Fragment
EDTA
PCR
Unlabeled Probes
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