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Une méthode efficace pour l'isolement de l'ARN hautement purifiées de semences pour une utilisation dans quantitative transcriptome Analyse
JoVE Journal
Biochemistry
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JoVE Journal Biochemistry
An Efficient Method for the Isolation of Highly Purified RNA from Seeds for Use in Quantitative Transcriptome Analysis

Une méthode efficace pour l'isolement de l'ARN hautement purifiées de semences pour une utilisation dans quantitative transcriptome Analyse

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06:31 min

January 11, 2017

DOI:

06:31 min
January 11, 2017

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Summary

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We have succeeded in establishing a method for RNA isolation from plant seeds containing large amounts of oils, proteins, and polyphenols, which have inhibitory effects on high-purity RNA isolation. Our method is suitable for monitoring the expression of genes with low level transcripts in seeds.

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