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1Photonics Group, Department of Physics,Imperial College London, 2Institute for Chemical Biology, Department of Chemistry,Imperial College London, 3MRC Clinical Sciences Centre,Hammersmith Hospital, 4Chemical Biology Section, Department of Chemistry,Imperial College London, 5Retroscreen Virology Ltd, 6Pfizer Global Research and Development,Pfizer Limited, Sandwich, Kent, UK, 7Centre for Histopathology,Imperial College London
Chapters
- 00:05Title
- 01:03Open Fluorescence Lifetime Imaging (FLIM)–High Content Analysis (HCA) Plugin Setup
- 01:51Instrument Response Function (IRF) Image and Reference Dye Data Acquisition
- 04:32Multiwell Plate Sample FLIM Data Acquisition
- 06:29Results: Representative FLIM Analyses in Cos and HeLa Cells
- 08:05Conclusion
Vi presenterer en åpen kildekode høy innholdsanalyse (HCA) instrument utnytte automatiserte fluorescens levetid imaging (FLIM) for analyse av protein interaksjoner bruker Förster resonans energi overføring (FRET) basert avlesninger. Datainnsamlingen for denne openFLIM-HCA instrument styres av programvare skrevet i μManager og dataanalyse er gjennomført i FLIMfit.
