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Developmental Biology
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母親の遺伝子産物の機能操作の使用<em>インビトロ</emゼブラフィッシュにおける>卵成熟
JoVE Journal
Developmental Biology
This content is Free Access.
JoVE Journal
Developmental Biology
Functional Manipulation of Maternal Gene Products Using
In Vitro
Oocyte Maturation in Zebrafish
Please note that all translations are automatically generated.
Click here for the English version.
母親の遺伝子産物の機能操作の使用<em>インビトロ</emゼブラフィッシュにおける>卵成熟
DOI:
10.3791/55213-v
•
10:39 min
•
April 22, 2017
•
Elaine L. Welch*
1
,
Celeste C. Eno*
1
,
Sreelaja Nair
,
Robin E. Lindeman
,
Francisco Pelegri
1
Laboratory of Genetics
,
University of Wisconsin-Madison
,
2
Department of Biological Sciences
,
Tata Institute of Fundamental Research
,
3
Department of Genetics, Cell Biology, and Development
,
University of Minnesota
Chapters
00:05
Title
00:50
Pre-selection of Females and Preparation of Maturation Medium
02:29
Dissection of Oocytes and Initiation of
In Vitro
Culture
04:56
Oocyte Microinjection
05:52
Oocyte Maturation and Defolliculation
07:18
Fertilization of
In Vitro
-cultured Oocytes
08:15
Results: Maternal Gene Product Manipulation Using
In Vitro
Oocyte Maturation
09:36
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
母方の遺伝子産物の操作に使用ゼブラフィッシュの卵母細胞の
体外
成熟のために最適化されたプロトコルは、ここに示されています。
Tags
In Vitro Oocyte Maturation
Zebrafish
Maternal Gene Products
Developmental Biology
Fertilization
Viable Embryos
Mating Tank
Leibovitz’s L-15 Medium
DHP
Gentamicin
Tricaine
Oocyte Dissection
Light Cycle
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