16,434 Views
•
10:50 min
•
February 25, 2017
DOI:
Summary
Automatically generated
We describe a method to sort single mammalian cells and to quantify the expression of up to 96 target genes of interest in each cell. This method includes the use of internal qPCR standards to enable the estimation of absolute transcript counts.
Related Videos
10:23
Single-cell Gene Expression Using Multiplex RT-qPCR to Characterize Heterogeneity of Rare Lymphoid Populations
Related Videos
10934 Views
09:25
Isolation and Characterization of Single Cells from Zebrafish Embryos
Related Videos
14519 Views
09:34
A Combinatorial Single-cell Approach to Characterize the Molecular and Immunophenotypic Heterogeneity of Human Stem and Progenitor Populations
Related Videos
6654 Views
10:20
Single-cell Profiling of Developing and Mature Retinal Neurons
Related Videos
14138 Views
06:26
Single Cell Analysis Of Transcriptionally Active Alleles By Single Molecule FISH
Related Videos
3130 Views
09:13
Gene Expression Profiling of Infecting Microbes Using a Digital Bar-coding Platform
Related Videos
8063 Views
08:37
Fluorescence Activated Cell Sorting (FACS) and Gene Expression Analysis of Fos-expressing Neurons from Fresh and Frozen Rat Brain Tissue
Related Videos
29269 Views
08:23
Single Cell Transcriptional Profiling of Adult Mouse Cardiomyocytes
Related Videos
17388 Views
13:06
Analysis of Single-cell Gene Transcription by RNA Fluorescent In Situ Hybridization (FISH)
Related Videos
15512 Views
07:00
Visualization and Analysis of mRNA Molecules Using Fluorescence In Situ Hybridization in Saccharomyces cerevisiae
Related Videos
34764 Views
Read Article
Cite this Article
Porter, J. R., Telford, W. G., Batchelor, E. Single-cell Gene Expression Profiling Using FACS and qPCR with Internal Standards. J. Vis. Exp. (120), e55219, doi:10.3791/55219 (2017).
Copy