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JoVE Journal
Developmental Biology

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Analyse af kromosomsegregation, histonacetylering og spindelmorfologi i hesteococytter
 

Analyse af kromosomsegregation, histonacetylering og spindelmorfologi i hesteococytter

Article doi: 10.3791/55242
May 11th, 2017

Summary May 11th, 2017

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Dette manuskript beskriver en eksperimentel tilgang til morfologisk og biokemisk karakterisering af hesteocyter. Specifikt illustrerer det foreliggende arbejde, hvordan man opsamler umodne og modne hesteocyter ved hjælp af ultralydstyret ægoptagelse (OPU) og hvordan man undersøger kromosomsegregation, spindelmorfologi, global histonacetylering og mRNA-ekspression.

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