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Pluripotent Verimli Farklılaşma NKX6-1 için Kök Hücreler<sup> +</sup> Pankreas Progenitörler
JoVE Journal
Developmental Biology
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JoVE Journal Developmental Biology
Efficient Differentiation of Pluripotent Stem Cells to NKX6-1+ Pancreatic Progenitors
DOI:

09:23 min

March 07, 2017

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Chapters

  • 00:05Title
  • 01:01HESC Differentiation and Flow Cytometry Analysis
  • 01:28Harvesting Cells for Flow Cytometry Analysis
  • 03:17Flow Preparation for Live Staining on Day 3
  • 04:41Flow Preparation for Fixed Staining on Day 13
  • 07:04Results: Schematic of Pancreatic Progenitor Differentiation from hESCs and Supporting Data
  • 08:26Conclusion

Summary

Automatic Translation

Burada in vitro NKX6-1 + pankreas atalarıdır insan embriyonik kök hücreleri ayırt etmek için 4 aşamalı bir protokol açıklar. Bu protokol, insan pluripotent kök hücre hatları çeşitli uygulanabilir.

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