Journal
/
/
系统抑制剂靶向细胞内的疗效和毒性筛选<em>结核分枝杆菌</em
JoVE Journal
Immunology and Infection
A subscription to JoVE is required to view this content.  Sign in or start your free trial.
JoVE Journal Immunology and Infection
System for Efficacy and Cytotoxicity Screening of Inhibitors Targeting Intracellular Mycobacterium tuberculosis
DOI:

09:57 min

April 05, 2017

,

Chapters

  • 00:05Title
  • 00:58THP-1 Medium and Maintenance and High-throughput Intracellular Screening Using Luciferase-expressing M. tuberculosis H37Rv
  • 03:54Cytotoxicity Analysis Using an MTT Assay
  • 06:06In-broth Activity Analysis Using a Resazurin Assay
  • 07:16Results: Efficacy Screening of Inhibitors of M. tuberculosis
  • 08:53Conclusion

Summary

Automatic Translation

我们已经开发了模块化高通量筛选系统,用于发现新化合物对结核分枝杆菌 ,靶向细胞内和细胞在肉汤中生长的细菌以及细胞毒性对哺乳动物宿主细胞

Related Videos

Read Article