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System til Effekt og cytotoksicitet Screening af inhibitorer Målretning Intracellulær<em> Mycobacterium tuberculosis</em
JoVE Journal
Immunology and Infection
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JoVE Journal Immunology and Infection
System for Efficacy and Cytotoxicity Screening of Inhibitors Targeting Intracellular Mycobacterium tuberculosis
DOI:

09:57 min

April 05, 2017

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Chapters

  • 00:05Title
  • 00:58THP-1 Medium and Maintenance and High-throughput Intracellular Screening Using Luciferase-expressing M. tuberculosis H37Rv
  • 03:54Cytotoxicity Analysis Using an MTT Assay
  • 06:06In-broth Activity Analysis Using a Resazurin Assay
  • 07:16Results: Efficacy Screening of Inhibitors of M. tuberculosis
  • 08:53Conclusion

Summary

Automatic Translation

Vi har udviklet en modulær højkapacitetsscreening system til at opdage hidtil ukendte forbindelser mod Mycobacterium tuberculosis, målretning intracellulære og i-bouillon voksende bakterier samt cytotoksicitet mod pattedyrværtscelle.

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