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System for Effekt og Cytotoksisitet Screening av inhibitorer målretting Intracellulær<em> Mycobacterium tuberculosis</em
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Immunology and Infection
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JoVE Journal Immunology and Infection
System for Efficacy and Cytotoxicity Screening of Inhibitors Targeting Intracellular Mycobacterium tuberculosis

System for Effekt og Cytotoksisitet Screening av inhibitorer målretting Intracellulær<em> Mycobacterium tuberculosis</em

DOI:
10.3791/55273-v

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09:57 min

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April 05, 2017

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,
1Department of Medicine,University of British Columbia

Chapters

  • 00:05Title
  • 00:58THP-1 Medium and Maintenance and High-throughput Intracellular Screening Using Luciferase-expressing M. tuberculosis H37Rv
  • 03:54Cytotoxicity Analysis Using an MTT Assay
  • 06:06In-broth Activity Analysis Using a Resazurin Assay
  • 07:16Results: Efficacy Screening of Inhibitors of M. tuberculosis
  • 08:53Conclusion

Summary

Automatic Translation

Automatic Translation

Vi har utviklet en modulær high-throughput screening system for å oppdage nye forbindelser mot Mycobacterium tuberculosis, rettet mot intracellulær og i-buljong voksende bakterier, så vel som cytotoksisitet mot pattedyrvertscellen.

Tags

Keywords: Mycobacterium TuberculosisIntracellular InfectionMacrophagesLuciferase AssayCytotoxicityMICDrug DevelopmentTHP1 CellsOpsonizationMOIDifferentiation96-well PlateCompound Screening

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