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Immunology and Infection
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系统抑制剂靶向细胞内的疗效和毒性筛选<em>结核分枝杆菌</em
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Immunology and Infection
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Immunology and Infection
System for Efficacy and Cytotoxicity Screening of Inhibitors Targeting Intracellular
Mycobacterium tuberculosis
Please note that all translations are automatically generated.
Click here for the English version.
系统抑制剂靶向细胞内的疗效和毒性筛选<em>结核分枝杆菌</em
DOI:
10.3791/55273-v
•
09:57 min
•
April 05, 2017
•
Xingji Zheng
,
Yossef Av-Gay
1
Department of Medicine
,
University of British Columbia
Chapters
00:05
Title
00:58
THP-1 Medium and Maintenance and High-throughput Intracellular Screening Using Luciferase-expressing
M. tuberculosis
H37Rv
03:54
Cytotoxicity Analysis Using an MTT Assay
06:06
In-broth Activity Analysis Using a Resazurin Assay
07:16
Results: Efficacy Screening of Inhibitors of
M. tuberculosis
08:53
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
我们已经开发了模块化高通量筛选系统,用于发现新化合物对
结核分枝杆菌
,靶向细胞内和细胞在肉汤中生长的细菌以及细胞毒性对哺乳动物宿主细胞
。
Tags
Mycobacterium Tuberculosis
Intracellular Infection
Macrophages
Luciferase Assay
Cytotoxicity
MIC
Drug Development
THP1 Cells
Opsonization
MOI
Differentiation
96-well Plate
Compound Screening
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