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Developmental Biology
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分离和新型表面标记组合来标识肝祖亚群富集
JoVE Journal
Developmental Biology
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JoVE Journal
Developmental Biology
Isolation and Enrichment of Liver Progenitor Subsets Identified by a Novel Surface Marker Combination
Please note that all translations are automatically generated.
Click here for the English version.
分离和新型表面标记组合来标识肝祖亚群富集
DOI:
10.3791/55284-v
•
08:52 min
•
February 18, 2017
•
Henrike Julich-Haertel
,
Marina Tiwari
,
Christina Mehrfeld
,
Elmar Krause
,
Miroslaw Kornek
,
Veronika Lukacs-Kornek
1
Department of Medicine II
,
Saarland University Medical Center
,
2
Department of Physiology, Center for Integrative Physiology and Molecular Medicine (CIPMM)
,
University of Saarland
Chapters
00:05
Title
00:58
Liver Single Cell Suspension Preparation
02:40
Flow Cytometric Cell Count Determination and Cell Surface Staining
04:30
Magnetic Microbead-based Progenitor Cell Enrichment
07:01
Results: Representative Liver Progenitor Subset Analyses
08:22
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
肝损伤都伴随着祖细胞扩张表示异质细胞群。这种蜂窝室的新颖分级允许多个子集的区别。此处所描述的方法示出了流式细胞术分析,并且可用于进一步的测定各种子集的高纯度分离的流动。
Tags
Liver Progenitor Cells
Liver Digestion Protocol
Flow Cytometry
Surface Marker Combination
Cell Isolation
Cell Biology
Hematopoietic Cells
Endothelial Cells
Mouse Liver
Cell Suspension
Cell Counting
Propidium Iodide
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