Journal
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L'induzione di una risposta infiammatoria in colture primarie epatociti di topi
JoVE Journal
Immunology and Infection
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JoVE Journal Immunology and Infection
Induction of an Inflammatory Response in Primary Hepatocyte Cultures from Mice
DOI:

08:32 min

March 10, 2017

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Chapters

  • 00:05Title
  • 00:54Preparation for Perfusion and Liver Recovery
  • 03:23Isolation and Treatment of Cells
  • 05:23Generating cDNA from Isolated RNA by Reverse Transcription and Analysis of Cells by Quantitative PCR
  • 06:32Results: Imflammatory Response in Mouse Primary Hepatocyte Cultures
  • 07:46Conclusion

Summary

Automatic Translation

Qui vi mostriamo un approccio enzimatico per isolare epatociti primari da topi adulti, e descriviamo la quantificazione di una risposta infiammatoria con ELISA e PCR in tempo reale.

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