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Immunology and Infection
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从小鼠在原代肝细胞培养物的炎性反应的诱导
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Immunology and Infection
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JoVE Journal
Immunology and Infection
Induction of an Inflammatory Response in Primary Hepatocyte Cultures from Mice
Please note that all translations are automatically generated.
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从小鼠在原代肝细胞培养物的炎性反应的诱导
DOI:
10.3791/55319-v
•
08:32 min
•
March 10, 2017
•
Brian Czaya*
1,2
,
Saurav Singh*
1,2
,
Christopher Yanucil
2
,
Karla Schramm
2
,
Christian Faul
2
,
Alexander Grabner
1
Katz Family Drug Discovery Center and Division of Nephrology and Hypertension, Department of Medicine
,
University of Miami Leonard M. Miller School of Medicine
,
2
Department of Cell Biology and Anatomy
,
University of Miami Leonard M. Miller School of Medicine
Chapters
00:05
Title
00:54
Preparation for Perfusion and Liver Recovery
03:23
Isolation and Treatment of Cells
05:23
Generating cDNA from Isolated RNA by Reverse Transcription and Analysis of Cells by Quantitative PCR
06:32
Results: Imflammatory Response in Mouse Primary Hepatocyte Cultures
07:46
Conclusion
Summary
Automatic Translation
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Automatic Translation
这里,我们表明酶促方法来原代肝细胞从成年小鼠隔离,我们描述了使用ELISA和实时PCR的炎症反应的量化。
Tags
Hepatocytes
Inflammatory Response
Cytokines
CRP
IL6
Hepatic Acute Phase Response
Hepatocellular Function
Hepatic Cytokines
Chronic Kidney Disease
Hepatic Metabolomics
Inflammation
Liver Perfusion
Liver Digest
IVC Cannulation
Liver Dissection
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