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分析细胞表面粘附重塑回应机械张力使用磁珠
JoVE Journal
Biology
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JoVE Journal Biology
Analyzing Cell Surface Adhesion Remodeling in Response to Mechanical Tension Using Magnetic Beads
DOI:

07:55 min

March 08, 2017

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Chapters

  • 00:05Title
  • 00:44Ligand Conjugation to Magnetic Beads
  • 02:58Application of Tensional Forces on the Ligand-coated Bead Bound Adhesion Receptors on the Dorsal Surface of Cells
  • 05:49Results: Mechanical Tension Induces Adhesion Maturation
  • 07:03Conclusion

Summary

Automatic Translation

细胞表面粘连是在机械传导的中心,因为它们传输机械张力,并开始参与组织稳态和发展的信号通路。这里,我们提出了解剖被响应于张力激活生化途径,使用包被配体 - 磁珠和施力到粘附受体的协议。

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