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分析细胞表面粘附重塑回应机械张力使用磁珠
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Analyzing Cell Surface Adhesion Remodeling in Response to Mechanical Tension Using Magnetic Beads
Please note that all translations are automatically generated.
Click here for the English version.
分析细胞表面粘附重塑回应机械张力使用磁珠
DOI:
10.3791/55330-v
•
07:55 min
•
March 08, 2017
•
Angélique Millon-Frémillon*
1
,
Julien Aureille*
1
,
Christophe Guilluy
1
Institute for Advanced Biosciences
,
Centre de recherche UGA – INSERM U1209 – CNRS UMR
Chapters
00:05
Title
00:44
Ligand Conjugation to Magnetic Beads
02:58
Application of Tensional Forces on the Ligand-coated Bead Bound Adhesion Receptors on the Dorsal Surface of Cells
05:49
Results: Mechanical Tension Induces Adhesion Maturation
07:03
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
细胞表面粘连是在机械传导的中心,因为它们传输机械张力,并开始参与组织稳态和发展的信号通路。这里,我们提出了解剖被响应于张力激活生化途径,使用包被配体 - 磁珠和施力到粘附受体的协议。
Tags
Magnetic Beads
Cell Surface Adhesion
Mechanical Tension
Biochemical Pathways
Mechano-transduction
Adhesion Receptors
Superparamagnetic Beads
Fibronectin
Cell Adhesion
BSA
Poly-D-lysine
Apotransferrin
PBS
Bovine Serum Albumin
Cell Culture
Adherent Cells
Article
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