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Kvalitativ og kvantitativ analyse af den immun Synapse i det menneskelige System, ved hjælp af Imaging flowcytometri
JoVE Journal
Immunology and Infection
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JoVE Journal Immunology and Infection
Qualitative and Quantitative Analysis of the Immune Synapse in the Human System Using Imaging Flow Cytometry
DOI:

08:35 min

January 07, 2019

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Chapters

  • 00:04Title
  • 00:38Pan-leukocyte Preparation and Immune Synapse Induction and Staining
  • 02:45Flow Cytometric Image Acquisition
  • 03:39Data Analysis
  • 05:54Results: Representative CD3 and F-actin Immune Synapse Enrichment Analysis
  • 07:22Conclusion

Summary

Automatic Translation

Her beskriver vi et komplet arbejdsgang for den kvalitative og kvantitative analyser af immun synapser mellem primære humane T celler og antigen-præsenterer celler. Metoden er baseret på imaging flowcytometri, som giver mulighed for erhvervelse og evaluering af flere tusinde celle billeder inden for en relativt kort periode.

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