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生物法测定抗 tnf 单克隆抗体 (抗体) 的相对效力
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Determination of the Relative Potency of an Anti-TNF Monoclonal Antibody (mAb) by Neutralizing TNF Using an
In Vitro
Bioanalytical Method
Please note that all translations are automatically generated.
Click here for the English version.
使用
体外
生物法测定抗 tnf 单克隆抗体 (抗体) 的相对效力
DOI:
10.3791/55376-v
•
16:07 min
•
September 16, 2017
•
Lilia Tierrablanca-Sánchez
,
Víctor Pérez Medina Martínez
,
Nancy D. Ramírez Ibañez
,
Néstor O. Pérez Ramírez
,
Francisco Luis Flores Ortiz
,
Emilio Medina-Rivero
1
Research and Development
,
Probiomed
,
2
Bioprocess Research and Development Unit
,
Instituto Politecnico Nacional
Chapters
00:00
Title
00:31
Introduction
02:32
Methodology: Solutions
02:51
Methodology: Cells Thawing and First Subculturing
04:33
Methodology: Verification of Confluency
05:21
Methodology: Second Subculturing, Cells Detaching and Counting
07:45
Methodology: Antibody Quantitation and Dilutions
09:37
Methodology: Neutralization Assay
13:00
Methodology: Spectrophotometric Analysis
13:54
Results: Dose Response Curve
15:23
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
本文介绍了一种利用 WEHI 164 细胞中和机制测定抗 TNFα单克隆人的相对亡活性的协议。本协议对于比较不同分子的中和强度具有相同的生物功能是有用的。
Tags
Anti-TNF Monoclonal Antibody
TNF-alpha Neutralization
In Vitro Bioanalytical Method
Relative Potency
WEHI 164 Cells
Apoptosis
Cell Culture
Cell Density
Antibody Dilution
Spectrophotometric Analysis
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