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使用基于酶的生物传感器来衡量进补和阶段性谷氨酸在阿尔茨海默氏症小鼠模型
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Neuroscience
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JoVE Journal Neuroscience
Using Enzyme-based Biosensors to Measure Tonic and Phasic Glutamate in Alzheimer’s Mouse Models
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使用基于酶的生物传感器来衡量进补和阶段性谷氨酸在阿尔茨海默氏症小鼠模型

DOI:
10.3791/55418-v

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10:46 min

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May 03, 2017

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, , , , ,
1Department of Psychology,West Virginia University, 2Department of Drug Discovery & Development,Auburn University, 3Department of Neuroscience,University of Kentucky Medical Center

Chapters

  • 00:05Title
  • 00:50Coating the MEA
  • 01:40Electroplating the Electrode
  • 02:46Preparing the Solutions Needed for Calibration
  • 04:48Assemble the Micropipette
  • 05:46General Animal Surgery for MEA Recordings
  • 09:01Results: Extracellular Tonic and Potassium Chloride-evoked Release of Glutamate in Hippocampus
  • 10:18Conclusion

Summary

Automatic Translation

Automatic Translation

在这里，我们描述了用于测量补药和采用酶联微电极阵列（MEA） 在体内阶段性细胞外谷氨酸的变化的空间和时间上精确的方法的设置，软件导航，和数据分析。

Tags

Enzyme-based BiosensorsTonic And Phasic GlutamateAlzheimer’s Mouse ModelsMicroElectrode ArraysNeurotransmitter LevelsNeurotransmitter Release And ClearanceSpatially And Temporally PreciseGlutamate OxidasePBSElectroplatingCalibrationHeating PadPBS Solution

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