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简捷方法对硫甙的提取与分析用高压液相色谱法(HPLC)
JoVE Journal
Chemistry
This content is Free Access.
JoVE Journal Chemistry
A Straightforward Method for Glucosinolate Extraction and Analysis with High-pressure Liquid Chromatography (HPLC)
DOI:

10:09 min

March 15, 2017

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Chapters

  • 00:05Title
  • 01:04Extraction Preparation
  • 04:03Glucosinolate Extraction and Analysis
  • 08:02Results: Identification and Quantification of Extracted Glucosinolates
  • 09:10Conclusion

Summary

Automatic Translation

Here, we describe in great detail an established and robust protocol for the extraction of glucosinolates from ground plant materials. After an on-column sulfatase treatment of the extracts, the desulfoglucosinolates are eluted and analyzed by high-pressure liquid chromatography.

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