Application de Codé génétiquement fluorescent oxyde nitrique (NO •) Sondes, les geNOps, pour l'imagerie en temps réel de NO • Les signaux dans les cellules simples
This manuscript presents protocols for the application of novel genetically encoded nitric oxide (NO•) probes (geNOps) to monitor single cell NO• fluctuations in real-time using fluorescence microscopy. The Ca2+-triggered NO• formation on the level of individual endothelial cells was visualized by combining geNOps with a chemical Ca2+ sensor.