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En IL-8 Transient Transgenized Mouse Model för<em> In vivo</em> Långvarig övervakning av inflammatoriska reaktioner
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1Department of Medicine, General Pathology Division, Cystic Fibrosis Translational Research Laboratory “D. Lissandrini”,University of Verona, 2Corporate Preclinical R&D,Chiesi Farmaceutici S.p.A., 3Department of Diagnostic and Public Health, Microbiology Division,University of Verona, 4Dipartimento di Scienze Medico-Veterinarie,University of Parma, 5Department of Biomedical Biotechnological and Translational Sciences,University of Parma, 6Department of Computer Science,University of Verona, 7Department of Neurological, Biomedical and Movement Sciences,University of Verona, 8Cystic Fibrosis Regional Center (CFC),AOUI Verona
Chapters
- 00:05Title
- 01:07In Vivo Gene Delivery and Biomluminescent Imaging (BLI) and Photon Quantitification
- 04:24Pro-inflammatory Stimuli Mouse Challenge
- 06:51Results: Representative P. aeruginosa-induced Lung Inflammation Supernatent In Vivo Imaging in IL-8 Transiently Trangenic Mice
- 07:42Conclusion
Metoden som beskrivs här möjliggör visualisering av IL-8-promotorberoende inflammationsaktivering i lungorna hos möss genom icke-invasiv bioluminescensavbildning (BLI). Samma djur kan utsättas för BLI flera gånger i upp till två månader från tiden för leverans av luciferasreporterkonstruktionen.
