JoVE
JoVE
Faculty Resource Center
Research
Behavior
Biochemistry
Biology
Bioengineering
Cancer Research
Chemistry
Developmental Biology
Engineering
Environment
Genetics
Immunology and Infection
Medicine
Neuroscience
JoVE Journal
JoVE Encyclopedia of Experiments
JoVE Chrome Extension
Education
Biology
Chemistry
Clinical
Engineering
Environmental Sciences
Pharmacology
Physics
Psychology
Statistics
JoVE Core
JoVE Science Education
JoVE Lab Manual
JoVE Quiz
JoVE Business
Videos Mapped to your Course
Authors
Librarians
High Schools
About
Sign-In
Sign In
Contact Us
Research
JoVE Journal
JoVE Encyclopedia of Experiments
Education
JoVE Core
JoVE Science Education
JoVE Lab Manual
High Schools
EN
EN - English
CN - 中文
DE - Deutsch
ES - Español
KR - 한국어
IT - Italiano
FR - Français
PT - Português
EN
EN - English
CN - 中文
DE - Deutsch
ES - Español
KR - 한국어
IT - Italiano
FR - Français
PT - Português
Close
Research
Behavior
Biochemistry
Bioengineering
Biology
Cancer Research
Chemistry
Developmental Biology
Engineering
Environment
Genetics
Immunology and Infection
Medicine
Neuroscience
Products
JoVE Journal
JoVE Encyclopedia of Experiments
Education
Biology
Chemistry
Clinical
Engineering
Environmental Sciences
Pharmacology
Physics
Psychology
Statistics
Products
JoVE Core
JoVE Science Education
JoVE Lab Manual
JoVE Quiz
JoVE Business
Videos Mapped to Your Course
Teacher Resources
Get in Touch
Instant Trial
Log In
EN
EN - English
CN - 中文
DE - Deutsch
ES - Español
KR - 한국어
IT - Italiano
FR - Français
PT - Português
Journal
/
Immunology and Infection
/
体外
和
活体
评估移植受体的 T、B 和髓细胞抑制活性和体液反应
JoVE Journal
Immunology and Infection
Author Produced
A subscription to JoVE is required to view this content.
Sign in or start your free trial.
JoVE Journal
Immunology and Infection
In Vitro
and
In Vivo
Assessment of T, B and Myeloid Cells Suppressive Activity and Humoral Responses from Transplant Recipients
Please note that all translations are automatically generated.
Click here for the English version.
体外
和
活体
评估移植受体的 T、B 和髓细胞抑制活性和体液反应
DOI:
10.3791/55510-v
•
18:48 min
•
August 12, 2017
•
Séverine Bézie
2
,
Claire Usal
2
,
Carole Guillonneau
2
1
Centre de Recherche en Transplantation et Immunologie UMR 1064
,
INSERM, Université de Nantes
,
2
Institut de Transplantation Urologie Néphrologie (ITUN)
,
CHU Nantes
Chapters
00:00
Title
00:37
LEW.1W to LEW.1A Allograft Procedure
02:18
Isolation of Allograft APCs (pDCs)
06:12
Isolation of Responder Effector T Cells and Regulatory T Cells (CD4
+
and CD8
+
CD45RC
low
)
10:30
CFSE Labeling of Responder Cells to Measure their Proliferation
12:09
FACS Staining and Analysis After 6 Days of Coculture
13:49
In Vivo
Assessment of the Cells Supressive Activity by Adoptive Cell Transfer in a Heart Grafted Recipient
16:33
Representative Results
18:03
Conclusion
Summary
Automatic Translation
English (Original)
العربية (Arabic)
中文 (Chinese)
Nederlands (Dutch)
français (French)
Deutsch (German)
עברית (Hebrew)
italiano (Italian)
日本語 (Japanese)
한국어 (Korean)
português (Portuguese)
русский (Russian)
español (Spanish)
Türkçe (Turkish)
Automatic Translation
在这里, 我们提出了一个诱导耐受性的移植, 并评估
体外
和
体内
的抑制能力的不同细胞亚群从接受者和免疫状态的受体向施主或外源性抗原。
Tags
Transplant
Tolerance
Regulatory Cells
Suppressive Capacity
Humoral Response
In Vitro
In Vivo
Splenocytes
Lewis Rat
Collagenase
EDTA
Cell Isolation
Article
Embed
ADD TO PLAYLIST
Usage Statistics
Related Videos
纯净小鼠的B细胞的诱导和评估类开关重组
T细胞受体在小鼠T细胞的逆转录病毒转导
piggyBac转座子
初级人类T细胞系统改造
荧光目标阵列的T细胞反应评价中的应用<em>在体内</em
对T细胞推导<em>在体外</em>从小鼠胚胎干细胞
测量<em>体外</em> HIV-1 Preintegration配合物的整合活动
的分离,分化和人类的定量分泌抗体的B细胞从血液:酶联免疫斑点作为体液免疫的功能读出
在
从幼稚的CD4
+
T细胞
的
人CD4
+
FOXP3
+
诱导调节性T细胞(iTregs的)使用含有TGF-β-协议的
体外
分化
巨噬细胞中含有吞噬的
鼠伤寒沙门氏菌
的分离
一种研究人体 T 细胞召回反应
的
吸水泡协议
Read Article