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In Vitro en In Vivo beoordeling van T-, B- en myeloïde cellen onderdrukkende activiteit en humorale reacties van transplantatie ontvangers
JoVE Journal
Immunology and Infection
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JoVE Journal Immunology and Infection
In Vitro and In Vivo Assessment of T, B and Myeloid Cells Suppressive Activity and Humoral Responses from Transplant Recipients
DOI:

18:48 min

August 12, 2017

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Chapters

  • 00:00Title
  • 00:37LEW.1W to LEW.1A Allograft Procedure
  • 02:18Isolation of Allograft APCs (pDCs)
  • 06:12Isolation of Responder Effector T Cells and Regulatory T Cells (CD4+ and CD8+CD45RClow)
  • 10:30CFSE Labeling of Responder Cells to Measure their Proliferation
  • 12:09FACS Staining and Analysis After 6 Days of Coculture
  • 13:49In Vivo Assessment of the Cells Supressive Activity by Adoptive Cell Transfer in a Heart Grafted Recipient
  • 16:33Representative Results
  • 18:03Conclusion

Summary

Automatic Translation

Hier presenteren we een protocol bij het opwekken van tolerantie in transplantatie, en beoordelen van in vitro en in vivo de onderdrukkende capaciteit van afzonderlijke cel subsets van de ontvanger en de immuunstatus van de ontvanger naar donor of exogene antigenen.

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