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Journal
/
Immunology and Infection
/
体外
と
体内
の T, B, 骨髄細胞の抑制活性と移植から体液性応答の評価
JoVE Journal
Immunology and Infection
Author Produced
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JoVE Journal
Immunology and Infection
In Vitro
and
In Vivo
Assessment of T, B and Myeloid Cells Suppressive Activity and Humoral Responses from Transplant Recipients
Please note that all translations are automatically generated.
Click here for the English version.
体外
と
体内
の T, B, 骨髄細胞の抑制活性と移植から体液性応答の評価
DOI:
10.3791/55510-v
•
18:48 min
•
August 12, 2017
•
Séverine Bézie
2
,
Claire Usal
2
,
Carole Guillonneau
2
1
Centre de Recherche en Transplantation et Immunologie UMR 1064
,
INSERM, Université de Nantes
,
2
Institut de Transplantation Urologie Néphrologie (ITUN)
,
CHU Nantes
Chapters
00:00
Title
00:37
LEW.1W to LEW.1A Allograft Procedure
02:18
Isolation of Allograft APCs (pDCs)
06:12
Isolation of Responder Effector T Cells and Regulatory T Cells (CD4
+
and CD8
+
CD45RC
low
)
10:30
CFSE Labeling of Responder Cells to Measure their Proliferation
12:09
FACS Staining and Analysis After 6 Days of Coculture
13:49
In Vivo
Assessment of the Cells Supressive Activity by Adoptive Cell Transfer in a Heart Grafted Recipient
16:33
Representative Results
18:03
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
ここでは、受信者からの明瞭な細胞のサブセットの抑制能力と受信者ドナーまたは外因性の抗原への免疫の状態、移植、耐性を誘導し、
in vitro
と
in vivo
評価プロトコルを提案します。
Tags
Transplant
Tolerance
Regulatory Cells
Suppressive Capacity
Humoral Response
In Vitro
In Vivo
Splenocytes
Lewis Rat
Collagenase
EDTA
Cell Isolation
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