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技术诱导和量化细胞衰老
JoVE Journal
Biology
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JoVE Journal Biology
Techniques to Induce and Quantify Cellular Senescence
DOI:

06:51 min

May 01, 2017

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Chapters

  • 00:05Title
  • 00:43DNA Damage-induced Senescence
  • 01:40Cell Staining for Senescence-associated (SA) Beta-Galactosidase
  • 03:30Quantifying SA Secretory Phenotype (SASP) Protein Levels
  • 04:37Results: Representative SA-beta-Galactosidase Staining and SASP Expression Analyses
  • 05:42Conclusion

Summary

Automatic Translation

细胞衰老,细胞周期停滞的不可逆状态,可通过各种细胞应激来诱导。在这里,我们描述的协议来诱导细胞衰老和方法来评估衰老的标志。

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