JoVE
JoVE
Faculty Resource Center
Research
Behavior
Biochemistry
Biology
Bioengineering
Cancer Research
Chemistry
Developmental Biology
Engineering
Environment
Genetics
Immunology and Infection
Medicine
Neuroscience
JoVE Journal
JoVE Encyclopedia of Experiments
JoVE Chrome Extension
Education
Biology
Chemistry
Clinical
Engineering
Environmental Sciences
Pharmacology
Physics
Psychology
Statistics
JoVE Core
JoVE Science Education
JoVE Lab Manual
JoVE Quiz
JoVE Business
Videos Mapped to your Course
Authors
Librarians
High Schools
About
Sign-In
Sign In
Contact Us
Research
JoVE Journal
JoVE Encyclopedia of Experiments
Education
JoVE Core
JoVE Science Education
JoVE Lab Manual
High Schools
EN
EN - English
CN - 中文
DE - Deutsch
ES - Español
KR - 한국어
IT - Italiano
FR - Français
PT - Português
EN
EN - English
CN - 中文
DE - Deutsch
ES - Español
KR - 한국어
IT - Italiano
FR - Français
PT - Português
Close
Research
Behavior
Biochemistry
Bioengineering
Biology
Cancer Research
Chemistry
Developmental Biology
Engineering
Environment
Genetics
Immunology and Infection
Medicine
Neuroscience
Products
JoVE Journal
JoVE Encyclopedia of Experiments
Education
Biology
Chemistry
Clinical
Engineering
Environmental Sciences
Pharmacology
Physics
Psychology
Statistics
Products
JoVE Core
JoVE Science Education
JoVE Lab Manual
JoVE Quiz
JoVE Business
Videos Mapped to Your Course
Teacher Resources
Get in Touch
Instant Trial
Log In
EN
EN - English
CN - 中文
DE - Deutsch
ES - Español
KR - 한국어
IT - Italiano
FR - Français
PT - Português
Journal
/
Biology
/
技术诱导和量化细胞衰老
JoVE Journal
Biology
A subscription to JoVE is required to view this content.
Sign in or start your free trial.
JoVE Journal
Biology
Techniques to Induce and Quantify Cellular Senescence
Please note that all translations are automatically generated.
Click here for the English version.
技术诱导和量化细胞衰老
DOI:
10.3791/55533-v
•
06:51 min
•
May 01, 2017
•
Nicole Noren Hooten
,
Michele K. Evans
1
Laboratory of Epidemiology and Population Science
,
National Institute on Aging, National Institutes of Health
Chapters
00:05
Title
00:43
DNA Damage-induced Senescence
01:40
Cell Staining for Senescence-associated (SA) Beta-Galactosidase
03:30
Quantifying SA Secretory Phenotype (SASP) Protein Levels
04:37
Results: Representative SA-beta-Galactosidase Staining and SASP Expression Analyses
05:42
Conclusion
Summary
Automatic Translation
English (Original)
العربية (Arabic)
中文 (Chinese)
Nederlands (Dutch)
français (French)
Deutsch (German)
עברית (Hebrew)
italiano (Italian)
日本語 (Japanese)
한국어 (Korean)
português (Portuguese)
русский (Russian)
español (Spanish)
Türkçe (Turkish)
Automatic Translation
细胞衰老,细胞周期停滞的不可逆状态,可通过各种细胞应激来诱导。在这里,我们描述的协议来诱导细胞衰老和方法来评估衰老的标志。
Tags
Cellular Senescence
Senescence Induction
Senescence Quantification
Gamma Irradiation
Senescence-associated Beta-galactosidase Assay
Cell Culture
Microscopy
Image Analysis
Article
Embed
ADD TO PLAYLIST
Usage Statistics
Related Videos
微机电与细胞生物学研究所:展望与应用
研究有丝分裂的显微注射技术
果蝇
合胞体胚胎
成像错配修复和DNA损伤的细胞反应
枯草芽孢杆菌</em
无菌实验室技术:电镀方法
RNAi筛选识别胚后的表型
C。线虫</em
端粒长度和端粒酶活性;阴阳细胞衰老
高级共焦显微镜技术研究 DNA 损伤的蛋白质-蛋白质相互作用和动力学
小鱼肾注射液循环系统中微粒的简易有效管理与可视化
鼻电位差量化小鼠跨上皮离子转运
时空映射和粒子分析技术在细胞内 ca
2 +
原位信令定量中的应用
Read Article