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Journal / Developmental Biology / Un dosage de point à 5 mC Quantification du…
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JoVE Journal Developmental Biology
Un dosage de point à 5 mC Quantification du niveau de méthylation d'ADN de la Dedifférenciation des chondrocytes
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Un dosage de point à 5 mC Quantification du niveau de méthylation d'ADN de la Dedifférenciation des chondrocytes

Article DOI: 10.3791/55565-v • 10:07 min • May 17th, 2017
May 17th, 2017 •
1,2, 3, 4,5, 2,6, 2, 2, 1,2
1Guangzhou Medical University, 2Shenzhen Key Laboratory of Tissue Engineering, Shenzhen Laboratory of Digital Orthopeadic Engineering, Department of Orthopedics, Shenzhen Second People's Hospital (The First Hospital Affiliated to Shenzhen University), 3Department of Chemistry, The Chinese University of Hong Kong, 4School of Biomedical Engineering, Shanghai Jiao Tong University, 5Renji Hospital Clinical Stem Cell Research Center, Shanghai Jiao Tong University School of Medicine, 6Shantou University Medical College

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Nous présentons une méthode pour quantifier la méthylation de l'ADN en fonction de la 5-méthylcytosine (5-mC) dot blot. Nous avons déterminé les niveaux de 5 mC pendant la dédifférenciation des chondrocytes. Cette technique simple pourrait être utilisée pour déterminer rapidement le phénotype des chondrocytes dans le traitement ACI.

Tags

Biologie du développement numéro 123 culture cellulaire expansion monocouche dédifférenciation des chondrocytes méthylation de l'ADN analyse par point blot 5-mC extraction de l'ADN génomique
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