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Un ensayo de transferencia de puntos de 5 mC cuantificando el nivel de metilación de ADN de la distredenciación de condrocitos<em> In Vitro</em
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1Guangzhou Medical University, 2Shenzhen Key Laboratory of Tissue Engineering, Shenzhen Laboratory of Digital Orthopeadic Engineering, Department of Orthopedics,Shenzhen Second People’s Hospital (The First Hospital Affiliated to Shenzhen University), 3Department of Chemistry,The Chinese University of Hong Kong, 4School of Biomedical Engineering,Shanghai Jiao Tong University, 5Renji Hospital Clinical Stem Cell Research Center,Shanghai Jiao Tong University School of Medicine, 6Shantou University Medical College
Chapters
- 00:05Title
- 01:52Human Articular Cartilage Tissue Collection and Chondrocyte Culture
- 03:33Genomic DNA Extraction
- 05:47Dot Blot nalysis
- 07:55Results: DNA Methylation Increases During Chondrocyte Dedifferentiation
- 08:45Conclusion
Se presenta un método para cuantificar la metilación del ADN basado en la 5-mctilcitosina (5-mC) dot blot. Se determinaron los niveles de 5-mC durante la desdiferenciación de condrocitos. Esta técnica simple podría utilizarse para determinar rápidamente el fenotipo de condrocito en el tratamiento de ACI.
