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Journal / Developmental Biology / 軟骨細胞分化のDNAメチル化レベルを定量化する5-mCドットブロットアッセイ…
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JoVE Journal Developmental Biology
軟骨細胞分化のDNAメチル化レベルを定量化する5-mCドットブロットアッセイ
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軟骨細胞分化のDNAメチル化レベルを定量化する5-mCドットブロットアッセイ

Article DOI: 10.3791/55565-v • 10:07 min • May 17th, 2017
May 17th, 2017 •
1,2, 3, 4,5, 2,6, 2, 2, 1,2
1Guangzhou Medical University, 2Shenzhen Key Laboratory of Tissue Engineering, Shenzhen Laboratory of Digital Orthopeadic Engineering, Department of Orthopedics, Shenzhen Second People's Hospital (The First Hospital Affiliated to Shenzhen University), 3Department of Chemistry, The Chinese University of Hong Kong, 4School of Biomedical Engineering, Shanghai Jiao Tong University, 5Renji Hospital Clinical Stem Cell Research Center, Shanghai Jiao Tong University School of Medicine, 6Shantou University Medical College

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本発明者らは、5-メチルシトシン（5-mC）ドットブロットに基づいてDNAメチル化を定量する方法を提示する。我々は、軟骨細胞脱分化の間の5-mCレベルを決定した。この単純な技術は、ACI治療における軟骨細胞の表現型を迅速に決定するために使用することができる。

Tags

発達生物学、第123号、細胞培養、単層拡大、軟骨細胞脱分化、DNAメチル化、5-mCドットブロット分析、ゲノムDNA抽出
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