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制备和<em>体外</em>磁化的表征的miR-改性内皮细胞
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Preparation and
In Vitro
Characterization of Magnetized miR-modified Endothelial Cells
Please note that all translations are automatically generated.
Click here for the English version.
制备和<em>体外</em>磁化的表征的miR-改性内皮细胞
DOI:
10.3791/55567-v
•
09:58 min
•
May 02, 2017
•
Natalia Voronina
,
Heiko Lemcke
,
Frank Wiekhorst
,
Jens-Peter Kühn
,
Markus Frank
,
Gustav Steinhoff
,
Robert David
1
Reference and Translation Center for Cardiac Stem Cell Therapy (RTC), Department of Cardiac Surgery
,
University of Rostock
,
2
Physikalisch-Technische Bundesanstalt
,
3
Department of Radiology and Neuroradiology
,
Ernst-Moritz-Arndt-University Greifswald
,
4
Electron Microscopy Center
,
University of Rostock
Chapters
00:05
Title
00:46
Transfection
02:38
Testing of Transfection Efficiency
04:21
Cell Targeting Evaluation and Magnetic Cell Separation
07:06
Results: Safe and Efficient Delivery of miRNA to Endothelial Cells and Their Magnetic Responsiveness
08:45
Conclusion
Summary
Automatic Translation
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Automatic Translation
这个手稿描述的miR的有效的,非病毒递送通过PEI / MNP矢量和它们的磁化与内皮细胞。因此,除了遗传修饰,该方法允许用于磁性细胞的指导和MRI可检测性。该技术可以被用来改善的治疗性细胞产品的特性。
Tags
Magnetized Cells
MiR-modified Cells
Endothelial Cells
Cell Magnetization
MicroRNA Transfection
Polyethyleneimene
Super Paramagnetic Iron Oxide Nanoparticles
Cell Therapy
Regenerative Medicine
Cell Retention
Cell Survival
Confocal Microscopy
Structured Illumination Microscopy
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