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Fluorescensbaseret neuraminidase-inhiberingsassay i vurdering af den følsomhed over influenzavira til neuraminidaseinhibitoren Class of Antivirale
JoVE Journal
Immunology and Infection
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JoVE Journal Immunology and Infection
Fluorescence-based Neuraminidase Inhibition Assay to Assess the Susceptibility of Influenza Viruses to The Neuraminidase Inhibitor Class of Antivirals

Fluorescensbaseret neuraminidase-inhiberingsassay i vurdering af den følsomhed over influenzavira til neuraminidaseinhibitoren Class of Antivirale

DOI:
10.3791/55570-v

•

09:31 min

•

April 15, 2017

•

,
1WHO Collaborating Centre for Reference and Research on Influenza,Peter Doherty Institute for Infection and Immunity

Chapters

  • 00:05Title
  • 01:00Determining the Neuraminidase Activity of Viruses
  • 02:29Assessing Virus Seceptibility to NA Inhibitors Using the NA Inhibition Assay
  • 05:18Calculation of IC50 Values
  • 07:01Results: Susceptibility of Influenza Viruses to the Neuraminidase Inhibitor Class of Antivirals
  • 08:55Conclusion

Summary

Automatic Translation

Automatic Translation

Vi beskriver anvendelsen af ​​et fænotypisk fluorescensbaseret neuraminidase inhiberingsassay at vurdere følsomheden af ​​influenza A og B virus til neuraminidaseinhibitoren klasse af antivirale lægemidler.

Tags

Fluorescence-based AssayNeuraminidase InhibitionInfluenza Virus SusceptibilityNeuraminidase Inhibitor AntiviralsMUNANAVirus TitrationNA Activity CurvesZanamivirOseltamivir CarboxylatePeramivirLaninamivir

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