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Chapters
- 00:05Title
- 00:41Thymidine- and Nocodazole-based (Thy-noc) Synchronization and Release
- 03:34HU-based Synchronization and Release from G1/S Boundary
- 05:14Collecting Samples for FACS Analysis and RNA Extraction
- 07:00RNA Extraction for Gene Expression Analysis
- 09:02Results: Thy-noc and HU Cell Synchronization Protocols Enable Analysis of Gene Expression During Specific Phases of the Cell Cycle
- 11:07Conclusion
Vi rapporterer to cellesynkroniseringsprotokoller, der giver en sammenhæng til at studere begivenheder relateret til specifikke faser af cellecyklussen. Vi viser, at denne tilgang er nyttig til analyse af reguleringen af specifikke gener i en upertureret cellecyklus eller ved udsættelse for midler, som påvirker cellecyklussen.
