/

/

肌网钙储备与细胞内舒张钙在心室肌细胞中的分离评价

JoVE Journal
Medicine

A subscription to JoVE is required to view this content. Sign in or start your free trial.

JoVE Journal Medicine

Assessment of Sarcoplasmic Reticulum Calcium Reserve and Intracellular Diastolic Calcium Removal in Isolated Ventricular Cardiomyocytes

Please note that all translations are automatically generated. Click here for the English version.

肌网钙储备与细胞内舒张钙在心室肌细胞中的分离评价

DOI:

10.3791/55797-v

•

11:00 min

•

September 18, 2017

•

Jing Gao*¹, Xiaolu Shi*², Hong He, Juhong Zhang, Ding Lin, Guosheng Fu, Dongwu Lai

¹Department of Cardiology, Sir Run Run Shaw Hospital,Zhejiang University School of Medicine, ²Experimental Research Center,China Academy of Chinese Medical Sciences, ³Department of Cardiology,The Third Hospital of Hangzhou City

Chapters

00:05Title
00:44Isolation of Left Ventricular Cardiomyocytes
04:15Set Up of Perfusion System
04:59Measurement of Intracellular Ca²⁺ Transient and Sarcomere Shortening
06:51Assessment of SR Ca²⁺ Reserve and Diastolic Ca²⁺ Removal Function
09:10Results: Assessment of SR Calcium Reserve and Calcium Removal Function
10:17Conclusion

Summary

Automatic Translation

细胞内钙循环对心肌细胞收缩和舒张功能的调节起着至关重要的作用。在这里, 我们描述了一个协议, 以评估肌网钙^{2 +}储备和舒张钙功能的心肌细胞钙显像系统。

Tags

Keywords: Sarcoplasmic Reticulum Calcium Reserve Intracellular Diastolic Calcium Removal Isolated Ventricular Cardiomyocytes Langendorff Perfusion NT Solution Calcium-free Tyrode Solution Collagenase A KB Solution Cell Isolation Cell Filtration Centrifugation

Article

Embed

ADD TO PLAYLIST

Usage Statistics

Related Videos

在分离的小鼠使用红桃心功能和能量学的评估³¹ P核磁共振

隔离与人类心室肌细胞的功能研究从新鲜手术样品

冠脉流量和冠状动脉血流储备用小鼠压力超负荷基于模型的超声评估

慢性血栓栓塞性肺动脉高压和右心室功能的仔猪评估

经胸斑点跟踪超声心动图左室心肌变形的定量评价

万古霉素滴入硫酸钙和自体骨治疗兔骨感染的改进模型

小鼠右心室舒张参数的超声心电图测量

小鼠右心室系统及缺氧诱发肺动脉高血压的侵入性血液动力学评估

生成心室样HiPSC衍生的心肌细胞和用于钙处理特征的高质量细胞制剂

使用皮肤心肌细胞进行心脏功能的体外评估

Read Article