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Journal
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Biology
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ODELAY:用于酵母生长多参数定量的大规模方法
JoVE Journal
Biology
This content is Free Access.
JoVE Journal
Biology
ODELAY: A Large-scale Method for Multi-parameter Quantification of Yeast Growth
Please note that all translations are automatically generated.
Click here for the English version.
ODELAY:用于酵母生长多参数定量的大规模方法
DOI:
10.3791/55879-v
•
11:19 min
•
July 03, 2017
•
Thurston Herricks
,
Fred D. Mast
2
,
Song Li
,
John D. Aitchison
2
1
Institute for Systems Biology
,
2
Center for Infectious Disease Research
Chapters
00:05
Title
00:39
Agar Mold Assembly
03:36
ODELAY Culture Preparation
05:41
Spotting on Agar Using Automated Liquid Spotting Robot
07:21
ODELAY Time-lapse Microscopy
09:03
Results: ODELAY Characterizes all 3 Growth Phase of Yeast Cells on Solid Media
10:00
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
我们提出了一种定量单个酵母细胞的生长表型的方法,它们使用称为酵母生活阵列的单细胞倍增评估(ODELAY)的延时显微镜在固体培养基上生长成菌落。可以直接观察和量化生长到菌落中的遗传相同细胞的群体异质性。
Tags
ODELAY
Multi-parameter Quantification
Yeast Growth
High-throughput
Population Heterogeneity
Agar Mold
Colony Forming Microorganism
Genetic Perturbations
Drug Interactions
Growth Phenotypes
Microbiology
Genetics
Systems Biology
Acrylic Mold
Glass Slide
Molten Agar
Mold Separation
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