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En enkel, Robust og høj overførselshastighed enkelt molekyle Flow strækker Assay gennemførelse for at studere Transport af molekyler langs DNA
JoVE Journal
Biochemistry
Author Produced
This content is Free Access.
JoVE Journal Biochemistry
A Simple, Robust, and High Throughput Single Molecule Flow Stretching Assay Implementation for Studying Transport of Molecules Along DNA
DOI:

12:05 min

October 01, 2017

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Chapters

  • 00:00Title
  • 00:15Introduction
  • 01:31Preparation of the biotin-λ-DNA
  • 02:52Coverslip functionalization
  • 04:10Flow cell construction
  • 05:44TIRF Imaging
  • 08:15Data Analysis
  • 11:41Conclusion

Summary

Automatic Translation

Denne protokol viser en enkel, robust og høj overførselshastighed enkelt molekyle flow-stretching assay for at studere endimensional (1D) udbredelse af molekyler langs DNA.

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