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Journal
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Biochemistry
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大肠癌细胞中磷酸化和微管结合与 Tau 蛋白定位的测定
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Biochemistry
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JoVE Journal
Biochemistry
Assay for Phosphorylation and Microtubule Binding Along with Localization of Tau Protein in Colorectal Cancer Cells
Please note that all translations are automatically generated.
Click here for the English version.
大肠癌细胞中磷酸化和微管结合与 Tau 蛋白定位的测定
DOI:
10.3791/55932-v
•
12:55 min
•
October 10, 2017
•
Md Nazmul Huda
2
,
Erdenedolgor Erdene-Ochir
2
,
Cheol-Ho Pan
2
1
Systems Biotechnology Research Center
,
KIST Gangneung Institute of Natural Products
,
2
Division of Bio-Medical Science & Technology, KIST School
,
Korea University of Science and Technology
Chapters
00:05
Title
01:01
Cell Culture, Treatment, and Examination of Protein Expression
02:53
Phosphate Assay
05:22
Microtubule-binding Assay
07:55
Localization and Expression of Tau After Treatment of Cells with Curcumin
10:23
Results: Phosphorylation, Microtubule Binding, and Localization of Tau Protein in Colorectal Cancer Cells
12:03
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
本手稿描述了测量 tau tau, 测量 tau 结合到微管的标准协议, 以及药物治疗后细胞内 tau 的定位。这些协议可以重复使用, 以筛选药物或其他化合物的目标 tau 或微管结合。
Tags
Tau Phosphorylation
Microtubule Binding
Tau Localization
Confocal Microscopy
Colorectal Cancer Cells
Phosphatase Assay
Microtubule-binding Assay
Tau Protein
HCT 116 Cells
Curcumin
Lithium Chloride
RIPA Buffer
Bradford Assay
Alkaline Phosphatase
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