JoVE
JoVE
Faculty Resource Center
Research
Behavior
Biochemistry
Biology
Bioengineering
Cancer Research
Chemistry
Developmental Biology
Engineering
Environment
Genetics
Immunology and Infection
Medicine
Neuroscience
JoVE Journal
JoVE Encyclopedia of Experiments
JoVE Chrome Extension
Education
Biology
Chemistry
Clinical
Engineering
Environmental Sciences
Pharmacology
Physics
Psychology
Statistics
JoVE Core
JoVE Science Education
JoVE Lab Manual
JoVE Quiz
JoVE Business
Videos Mapped to your Course
Authors
Librarians
High Schools
About
Sign-In
Sign In
Contact Us
Research
JoVE Journal
JoVE Encyclopedia of Experiments
Education
JoVE Core
JoVE Science Education
JoVE Lab Manual
High Schools
EN
EN - English
CN - 中文
DE - Deutsch
ES - Español
KR - 한국어
IT - Italiano
FR - Français
PT - Português
EN
EN - English
CN - 中文
DE - Deutsch
ES - Español
KR - 한국어
IT - Italiano
FR - Français
PT - Português
Close
Research
Behavior
Biochemistry
Bioengineering
Biology
Cancer Research
Chemistry
Developmental Biology
Engineering
Environment
Genetics
Immunology and Infection
Medicine
Neuroscience
Products
JoVE Journal
JoVE Encyclopedia of Experiments
Education
Biology
Chemistry
Clinical
Engineering
Environmental Sciences
Pharmacology
Physics
Psychology
Statistics
Products
JoVE Core
JoVE Science Education
JoVE Lab Manual
JoVE Quiz
JoVE Business
Videos Mapped to Your Course
Teacher Resources
Get in Touch
Instant Trial
Log In
EN
EN - English
CN - 中文
DE - Deutsch
ES - Español
KR - 한국어
IT - Italiano
FR - Français
PT - Português
Journal
/
Chemistry
/
多肽和蛋白质量化使用自动免疫-MALDI (iMALDI)
JoVE Journal
Chemistry
A subscription to JoVE is required to view this content.
Sign in or start your free trial.
JoVE Journal
Chemistry
Peptide and Protein Quantification Using Automated Immuno-MALDI (iMALDI)
Please note that all translations are automatically generated.
Click here for the English version.
多肽和蛋白质量化使用自动免疫-MALDI (iMALDI)
DOI:
10.3791/55933-v
•
08:57 min
•
August 18, 2017
•
Huiyan Li
,
Robert Popp
,
Bjorn Frohlich
,
Michael X. Chen
,
Christoph H. Borchers
3,4,5
1
University of Victoria-Genome BC Proteomics Centre
,
2
Jewish General Hospital
,
McGill University
,
3
Dept of Biochemistry and Microbiology
,
University of Victoria
,
4
Proteomics Centre, Segal Cancer Centre, Lady Davis Institute, Jewish General Hospital
,
McGill University
,
5
Gerald Bronfman Department of Oncology
,
Jewish General Hospital
Chapters
00:05
Title
01:06
Generation of Ang I in Human Plasma
03:01
Immuno-enrichment of Ang I Using Antibody-coated Beads
06:04
Transfer of Beads onto MALDI Target Plate and MALDI-TOF-MS Analysis
06:55
Results: Quantification of Ang I Using iMALDI
07:53
Conclusion
Summary
Automatic Translation
English (Original)
العربية (Arabic)
中文 (Chinese)
Nederlands (Dutch)
français (French)
Deutsch (German)
עברית (Hebrew)
italiano (Italian)
日本語 (Japanese)
한국어 (Korean)
português (Portuguese)
русский (Russian)
español (Spanish)
Türkçe (Turkish)
Automatic Translation
提出了在使用自动的免疫-MALDI (iMALDI) 技术的复杂生物体液蛋白质定量的协议。
Tags
Peptide Quantification
Protein Quantification
Immuno-MALDI
Automated Assay
Plasma Samples
Angiotensin I
Antibody Conjugation
Protein Biomarkers
Quantitative Proteomics
Article
Embed
ADD TO PLAYLIST
Usage Statistics
Related Videos
生产二硫键稳定的跨膜肽复合物的结构研究
复用荧光微阵列人类唾液蛋白质分析用高分子微球和光纤束
Structure and Coordination Determination of Peptide-metal Complexes Using 1D and 2D
1
H NMR
使用氢交换质谱分析蛋白质动力学
分裂和池合成及肽叔酰胺图书馆表征
一个主干环肽库作为潜在的抗寄生虫治疗微波辐射的发展
CN-GELFrEE - 透明天然凝胶洗脱液体馏分偷天陷阱电泳
表征,气相炭用苯聚羧酸的定量和特定化合物同位素分析(BPCA)
毛细管电泳监视肽接枝壳聚糖膜实时
原位
金属氧化物纳米粒子的核探针检测与单细胞定量研究
Read Article