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基于磁珠复合法的撕裂细胞因子分析
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Immunology and Infection
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JoVE Journal Immunology and Infection
Bead Based Multiplex Assay for Analysis of Tear Cytokine Profiles
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基于磁珠复合法的撕裂细胞因子分析

DOI:
10.3791/55993-v

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09:22 min

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October 13, 2017

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, , , , , ,
1National Healthcare Group Eye Institute,Tan Tock Seng Hospital, 2Singapore Eye Research Institute (SERI), 3Institute of Molecular and Cell Biology,A*STAR, 4GROW Research Laboratory,Narayana Nethralaya Foundation, 5Vimta Labs Limited

Chapters

  • 00:05Title
  • 00:42Elution of Tear Sample from Tear Flow Strip
  • 01:26Cytokine Profiling by Bead Based Multiplex Assay
  • 04:43Reading the Assay Plate and Data Analysis
  • 06:56Results: Tear Cytokine Profiles from Four Male Subjects
  • 08:54Conclusion

Summary

Automatic Translation

Automatic Translation

泪膜细胞因子的分析有助于研究各种眼部疾病。基于微珠的复合检测方法简单、灵敏, 能够对小体积样品中的多个目标进行测试。在这里, 我们描述了一个协议的撕裂膜细胞因子分析一个使用珠基复用法。

Tags

Tear Cytokine ProfileBead-based Multiplex AssaySchirmer StripOphthalmologyCytokinesOcular DiseasesAnalytesTear SampleAssay BufferCentrifugationCytokine ProfilingMultiplex AssayCytokine StandardsAssay WorksheetMagnetic Plate Washer

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