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Journal
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Biochemistry
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mRNA从植物原生质体捕获
JoVE Journal
Biochemistry
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JoVE Journal
Biochemistry
mRNA Interactome Capture from Plant Protoplasts
Please note that all translations are automatically generated.
Click here for the English version.
mRNA从植物原生质体捕获
DOI:
10.3791/56011-v
•
12:29 min
•
July 28, 2017
•
Zhicheng Zhang
,
Kurt Boonen
,
Meixia Li
,
Koen Geuten
1
Department of Biology
,
KU Leuven
Chapters
00:05
Title
01:15
In Vivo
mRNA-protein Crosslinking by UV Irradiation
02:38
Protoplast Lysis Under Denaturing Conditions
03:59
mRNP Pull-down and Purification by Oligo-d(T)
25
Beads
08:14
mRBP Concentration and Protein Assays
10:04
Results: Capture of UV-crosslinked mRNA-bound Proteins by Oligo-d(T)
25
Beads
11:53
Conclusion
Summary
Automatic Translation
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Automatic Translation
在这里,我们提出了应用于
拟南芥
叶肉叶肉原生质体的相互作用捕获方案。该方法严格依赖
于体内
紫外线交联,并允许从生理环境中分离和鉴定植物mRNA结合蛋白。
Tags
MRNA Interactome
MRNA-bound Proteome
Plant Protoplasts
Post-transcriptional Gene Regulation
UV Crosslinking
Arabidopsis Thaliana
Leaf Mesophyll Protoplasts
MMG Solution
Centrifugation
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