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患者胶质母细胞瘤切除中细胞微环境的定量免疫组织化学

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Medicine

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JoVE Journal Medicine

Quantitative Immunohistochemistry of the Cellular Microenvironment in Patient Glioblastoma Resections

Please note that all translations are automatically generated. Click here for the English version.

患者胶质母细胞瘤切除中细胞微环境的定量免疫组织化学

DOI:

10.3791/56025-v

•

05:45 min

•

July 31, 2017

•

Jessica X. Yuan, Jennifer M. Munson

¹Biomedical Engineering,University of Virginia

Chapters

00:05Title
00:58FFPE Sample Deparaffinization and Rehydration and Antigen Retrieval
01:54Chromogenic Immunohistochemistry
03:50Results: Staining Effective for Oligodendrocyte Quantification
05:17Conclusion

Summary

Automatic Translation

该方案被开发用于使用显色免疫组织化学和ImageJ定量鉴定成胶质细胞瘤患者切除中的肿瘤微环境成分。

Tags

Keyword Extraction:Quantitative Immunohistochemistry Cellular Microenvironment Glioblastoma Resections Tumor Microenvironment Chromogenic Immunohistochemistry Image Analysis Antigen Retrieval Permeabilization Blocking Primary Antibodies Horseradish Peroxidase Diaminobenzidine

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