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测定野生型肝脏和牛奶样品中的唾液酸<em> CMAH</em>敲出小鼠
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Biology
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JoVE Journal
Biology
Determination of Sialic Acids in Liver and Milk Samples of Wild-type and
CMAH
Knock-out Mice.
Please note that all translations are automatically generated.
Click here for the English version.
测定野生型肝脏和牛奶样品中的唾液酸<em> CMAH</em>敲出小鼠
DOI:
10.3791/56030-v
•
08:04 min
•
July 14, 2017
•
Cui Cao
,
Wen J. Wang
,
Ying Y. Huang
,
Hong L. Yao
,
Louis P. Conway
,
Li Liu
,
Josef Voglmeir
1
Glycomics and Glycan Bioengineering Research Center (GGBRC), College of Food Science and Technology
,
Nanjing Agricultural University
Chapters
00:05
Title
00:55
Isolation of Sialic Acids from Milk and from Liver Tissue
03:26
Preparation of a Mixed Sialic Acid Standard
04:26
Fluorescence Derivatization of Sialic Acids
05:18
HPLC Analysis of Sialic Acid Derivatives
06:15
Results: Quantification of Sialic Acids in Liver and Milk Samples of Wild-type and CMAH Knock-out Mice
07:05
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
我们描述了一种基于HPLC的方法,用于测定小鼠肝脏和牛奶中的N-乙酰神经氨酸和N-羟乙酰神经氨酸。
Tags
Sialic Acid
N-Acetylneuraminic Acid
N-Glycolylneuraminic Acid
CMAH
Liver
Milk
HPLC
Glycoscience
Mouse Model
Sample Preparation
Anion Exchange Chromatography
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