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JoVE Journal Biology
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In Vivo Détection et analyse des Rb SUMOylation de protéines dans les cellules humaines

Article DOI: 10.3791/56096
November 2nd, 2017 •
*1,2, *1,2, 1,2, 1,2
1Department of Ophthalmology, Eye and ENT Hospital of Fudan University, 2Shanghai Key Laboratory of Visual Impairment and Restoration, Fudan University
* These authors contributed equally

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November 2nd, 2017

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Protéines de la famille petit axés sur l’ubiquitine modificateur (SUMO) sont conjugués aux résidus lysine de protéines cibles pour réglementer divers processus cellulaires. Cet article décrit un protocole pour la détection de la protéine du rétinoblastome (Rb) SUMOylation conditions endogènes et exogènes dans les cellules humaines.

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