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JoVE Journal Biology
I Vivo Deteksjon og analyse av Rb Protein SUMOylation i menneskeceller
Pulse-chase Analysis of N-linked Sugar Chains from Glycoproteins in Mammalian Cells
Journal (Biology)
Pulse-chase Analysis of N-linked Sugar Chains from Glycoproteins in Mammalian Cells
Bromodeoxyuridine (BrdU) Labeling and Subsequent Fluorescence Activated Cell Sorting for Culture-independent Identification of Dissolved Organic Carbon-degrading Bacterioplankton
Journal (Biology)
Bromodeoxyuridine (BrdU) Labeling and Subsequent Fluorescence Activated Cell Sorting for Culture-independent Identification of Dissolved Organic Carbon-degrading Bacterioplankton
 
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I Vivo Deteksjon og analyse av Rb Protein SUMOylation i menneskeceller

Article DOI: 10.3791/56096
November 2nd, 2017 •
*1,2, *1,2, 1,2, 1,2
1Department of Ophthalmology, Eye and ENT Hospital of Fudan University, 2Shanghai Key Laboratory of Visual Impairment and Restoration, Fudan University
* These authors contributed equally

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November 2nd, 2017

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Små ubiquitin-relaterte modifikator (SUMO) familie proteiner er konjugert til lysin rester av målet proteiner til å regulere ulike cellulære prosesser. Dette dokumentet beskriver en protokoll for påvisning av retinoblastoma (Rb) protein SUMOylation under endogene og ytre forhold i menneskeceller.

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