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在体内
人细胞 Rb 蛋白 sumo 的检测与分析
JoVE Journal
Biology
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JoVE Journal
Biology
In Vivo
Detection and Analysis of Rb Protein SUMOylation in Human Cells
Please note that all translations are automatically generated.
Click here for the English version.
在体内
人细胞 Rb 蛋白 sumo 的检测与分析
DOI:
10.3791/56096-v
•
09:40 min
•
November 02, 2017
•
Fengxi Meng*
1,2
,
Xiaofeng Li*
1,2
,
Hui Ren
2
,
Jiang Qian
2
1
Department of Ophthalmology
,
Eye and ENT Hospital of Fudan University
,
2
Shanghai Key Laboratory of Visual Impairment and Restoration
,
Fudan University
Chapters
00:05
Title
00:54
Cell Culture and Cell Cycle Synchronization
03:18
Immunoprecipitation of Endogenerous Rb Protein
06:12
Analysis of 6XHis-tagged Exogenous Rb SUMOylation in Human Cells
08:20
Results: Detection of Both Endogenous and Exogenous Rb SUMOylation
09:08
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
小泛素相关的修饰剂 (相扑) 家族蛋白被共轭到靶蛋白的赖氨酸残留物来调节各种细胞过程。本文介绍了一种检测视网膜母细胞瘤 (Rb) 蛋白 sumo 的方法。
Tags
Rb Protein SUMOylation
In Vivo Detection
Human Cells
SUMO Conjugation
Cell Synchronization
G1 Phase
S Phase
G2/M Phase
HEK293 Cells
Cell Cycle Analysis
Flow Cytometry
RIPA Lysis
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