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干膜 Photoresist-based 电化学微流控生物传感器平台: 器件制备、片上检测制备及系统运行
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Bioengineering
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JoVE Journal Bioengineering
Dry Film Photoresist-based Electrochemical Microfluidic Biosensor Platform: Device Fabrication, On-chip Assay Preparation, and System Operation
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干膜 Photoresist-based 电化学微流控生物传感器平台: 器件制备、片上检测制备及系统运行

DOI:
10.3791/56105-v

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13:42 min

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September 19, 2017

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, , ,
1Department of Microsystems Engineering,University of Freiburg, 2Freiburg Materials Research Center,University of Freiburg, 3Department of Biosystems Science and Engineering,ETH Zurich

Chapters

  • 00:05Title
  • 01:11Fabrication of the Microfluidic Biosensor Using Dry Film Photoresist (DFR) Technology
  • 07:29On-chip Assay Immobilization Procedure
  • 09:34Amperometric Signal Detection Using the Stop-flow Technique
  • 11:12Results: Working Principle of the Biosensor and the Signal Readout
  • 12:26Conclusion

Summary

Automatic Translation

Automatic Translation

利用低成本干膜光刻胶技术对各种分析进行快速、灵敏的定量化设计, 制备了微流控生物传感器平台。这种一次性系统允许通过停止流动技术在片上固定的酶联用的电化学读出。

Tags

AI-based BiosensorElectrochemical BiosensorDry Film PhotoresistMicrofluidic PlatformPoint-of-care TestingAnalyte QuantificationLow-cost FabricationVersatile Application

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